An idea that I decided craft up, following a now-removed
sciencefurs submission (alternative link here).
sciencefurs submission (alternative link here).
Category Artwork (Digital) / Comics
Species Canine (Other)
Size 1056 x 525px
File Size 167.1 kB
Its so true for biology. :s I've been running gels for months and getting craptastic results. Although, when doing experiments involving fluorescence and isothermal calorimetry, which is a bit more analytical chemistry and physical chemistry applied to biological problems, I get a wonderful data and the experiments work... then I have to analyze the results on a computer for days...
I get that. There seems to be this massive imbalance between setup and analysis when it comes to experiments, even when they are working perfectly (or nearly enough to it). For example, my real time PCRs are reasonably quick to set up (72-well experiments set up in just under an hour), but then I spend at least double that time analysing that data (I'm trying to find rare sequence variants in a gene, which get detected as a changing in amplicon melting behaviour and as such there tend to be quite a few false positives). Compare that microarrays: there is a crapton of work needed to get the results (all the way from tissue culture to running the array to processing and QCing the data for the actual analysis), then about 5-10 seconds worth of actually looking at the final results.
Our biology department here isn't good enough to do real time PCRs so most of my research is done in the chemistry department. I typically use various dyes and fluorescence quenching, absorbance, all kinds of electrochemistry, ITC, and I've only recently gotten a chance to even just run a few basic gels. Trying to convince my research advisers to let me have a human tissue culture, but we only have one laminar hood and we can hardly keep the E. coli living let alone a eukaryote culture. No one uses it though because chemistry owns it.
Also, YAY furry science talk.
Also, YAY furry science talk.
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